High Fidelity PCR Reagents and Kits
New England Biolabs, Inc. Phusion™ High-Fidelity DNA Polymerase – 100 units
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration).
VAZYME INTERNATIONAL LLC Ultra-high fidelity PCR Mix
Phanta Ultra Master Mix is a next-generation high-fidelity PCR reagent developed on the BioSmart platform, delivering ultra-high fidelity, rapid amplification at 5 sec/kb, and broad Tm compatibility. With advanced hot-start technology and an optimized buffer system, it ensures high success rate and excellent specificity at universal annealing temperature, enabling amplification of fragments up to 40 kb. Featuring fidelity up to 200-fold higher than Taq polymerase, it is ideal for molecular cloning, sequencing, site-directed mutagenesis, and other applications requiring highly accurate DNA amplification. Phanta Ultra Master Mix provides a fast, reliable, and convenient solution for high-performance PCR workflows.
Sigma Aldrich Fine Chemicals Biosciences ExpandTM High fidelity PCR
The 5 - 3 polymerase activity of Taq DNA Polymerase and the 3 - 5 exonuclease of the proofreading polymerase combine for improved performance and results 3 - 5 exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
Bio Rad Laboratories IPROOF HF MASTER MIX
Mix, Master; Bio-Rad; iProof HF; For high fidelity templates; 100 x 50uL reactions; Includes 2X master mix (0.04 U/uL), DMSO
Roche Diagnostics KAPA HiFi HotStart PCR Kit (250 U), 250 x 50 uL reactions
KAPA HiFi HotStart PCR Kit (250 U), 250 x 50 uL reactions. *This item is non returnable.
Bio Rad Laboratories .PROOF HIGHFIDELITY DNA POLYME
DNA, Polymerase; Bio-Rad; iProof High-Fidelity; 2 U/muL, 100 U; Includes: 5x reaction buffers, MgCl2 solution, DMSO
LGC GENOMICS LLC (Lucigen) FailSafe PCR 2X PreMix L, 2.5 mLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.
Trusted: The same great performance as the FailSafe PCR System, but available as individual componentsFlexible: Purchase any combination of FailSafe 2X PreMixesThis listing is only for reordering of individual FailSafe PCR 2X PreMixes.Before purchasing these separate components, we strongly recommend first optimizing your PCR conditions using the FailSafe PCR PreMix Selection Kit (Item ID. FS99060) in order to know which components to purchase.
New England Biolabs, Inc. Phusion™ High-Fidelity PCR Master Mix with HF Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Phusion High-Fidelity PCR Kit – 50 reactions
Phusion High-Fidelity DNA Polymerase offers both high-fidelity and robust performance, and thus can be used for all PCR applifications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. The Phusion High-Fidelity PCR Kit contains a sufficient supply of Phusion High-Fidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions or 200 reactions.
New England Biolabs, Inc. Q5 High-Fidelity PCR Kit – 50 reactions
Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions or 200 reactions. For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
Bio Rad Laboratories IPROOF HIGHFIDELITY PCR KIT
Kit, iProof High-Fidelity PCR; Bio-Rad; Size: Size: 2 U/uL; 50 U; Includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, lambda DNA, 1.3 and 10 kb primers, DNA standard
Takara Bio ADVANTAGE 2 PCR ENZYME SYSTEM
Advantage 2 PCR Enzyme Kit, 30 rxns, An optimized PCR enzyme mix for use in applications that involve longer templates or require increased fidelity and higher PCR performance
LGC GENOMICS LLC (Lucigen) FAILSAFE PCR PREMIX C 2.5MLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.
Trusted: The same great performance as the FailSafe PCR System, but available as individual componentsFlexible: Purchase any combination of FailSafe 2X PreMixesThis listing is only for reordering of individual FailSafe PCR 2X PreMixes.Before purchasing these separate components, we strongly recommend first optimizing your PCR conditions using the FailSafe PCR PreMix Selection Kit (Item ID. FS99060) in order to know which components to purchase.
New England Biolabs, Inc. Phusion High-Fidelity PCR Master Mix with GC Buffer – 500 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with GC Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.
New England Biolabs, Inc. Phusion™ High-Fidelity PCR Master Mix with HF Buffer – 100 reactions (50 µl vol)
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerase available. It possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2.